Journal: Pathogens

Article Title: An Evaluation of IL-10 Encoded by Cytomegalovirus in the Prediction of Coronary Artery Disease in People Living with HIV

doi: 10.3390/pathogens15020192

Figure Lengend Snippet: Sequence alignments of IL-10 encoded by laboratory CMV strains (2–7), Epstein–Barr Virus (8. EBV), humans (9) and mice (10). The selected cmvIL-10 peptide is included (1) and the perfect match with all CMV strains analysed is highlighted by a red box. Colours denote amino acid variations from the consensus shown in lines 2–7. The alignment was performed using Geneious alignment tool with default parameters. The image was generated using Geneious version 2025.2 created by Biomatters, Auckland, New Zealand < https://www.geneious.com >. Protein sequences were sought from NCBI. Accession numbers are in .

Article Snippet: Binding was detected using 0.2 μg/mL biotinylated polyclonal goat anti-cmvIL-10 antibody (BAF117, R&D Systems) for 2 h. After further washes, 1:2000 streptavidin-conjugated HRP (BD Biosciences, Franklin Lakes, NJ, USA) diluted in 1% BSA/PBS was added for 30 min. Plates were washed and 3,3′-5,5′ tetramethylbenzidine (TMB) substrate (Sigma–Aldrich, St. Louis, MO, USA) was added, reactions were stopped with 1M H 2 SO 4 and read at 450 nm.

Techniques: Sequencing, Virus, Generated